TABLE 1.
Ad5 sequences used for cloning regulatory and structural genesa
| Viral DNA | Start codon (bp) | Stop codon (bp) | Map units (kbp) | Transcription unit |
|---|---|---|---|---|
| pTP | 10544 | 8585 | 28.8–23.4 | l-strand |
| DNA-Pol | 8367 | 5200 | 22.8–14.1 | l-strand |
| DBP | 24032 | 22446 | 61.6–66.5 | l-strand |
| Hexon | 18842 | 21700 | 51.6–59.7 | r-strand |
| Penton | 14156 | 15870 | 39.3–44.2 | r-strand |
| Fiber | 31043 | 32790 | 86.4–91.3 | r-strand |
a
Ad5 ORFs used for cloning into the vaccinia virus expression vector pSCII. The precise positions of start and stop codons from Ad5 regulatory genes (pTP, DNA-Pol, and DBP) and structural genes (hexon, penton, and fiber) as well as the map units within the 35,935-bp Ad5 genome and the transcription unit used for transcribing the gene are indicated.