TABLE 1.
Oligonucleotide primers used in PCR
| Primer | Sequencea |
|---|---|
| VP4LZF | GACGGGGATCCAGGTTCATCGGACTGCGCGTCGAA |
| VP4LZR | CGGCTGCAGTTACAAATCCAATCCCATCCGTTT |
| VP4LZP | CGGCTGCAGTTACAAATCCAATCCCATCCGTTTCAG TTCGTCAGCCGTCTCATGTGGTGTTGG |
| AvaII F(Pr) | TGTCTGAGGGTCCGAAACCCAACACCACATGAG |
| CD2VP4R | GATAATCTGCAGCTATTTCCGTTTGTATTCCCTT |
| BINF | AACTGCAGATGGACGCTTCCGTGACGAAAGTT |
| AvaII R | GGTTTCGGACCCTCAGCACGGAT |
| AvaII F(Al) | TGTCTGAGGGTCCGAAACCCAACAGCGCATGAG |
a
Restriction enzyme sites are underlined, and mutated sequences are shown in boldface type.