Figure 2.

Effect of severe osmotic stress (PEG15%) on seedling biomass. (A) Comparative leaf and root phenotype in two-week stressed and non-stressed seedlings. Bar = 10 cm. (B,C) Measurement of fresh (B) and dry (C) weight of seedlings in (A). (D) Measurement of fresh root weight. (E–G) Relative weight of fresh seedlings (E), dry seedlings (F), and roots (G) after PEG treatment by normalizing with mock in each genotype. Error bars indicate the means ± SD (n = 15) of three biological replicates. Asterisks specify a significant difference, whereas “ns” denotes non-significant, obtain via two-way ANOVA (Tukey’s test), where **** p < 0.0001, *** p < 0.001, ** p < 0.01, and * p < 0.05.