FIG. 6.

In vitro transcription of pe38/polyhedrin hybrid promoters. In vitro transcription of the hybrid promoters pPE38muTAAG/FR, pPE38muTAAG/F, pPE38muTAAG/R, pPE38muTAAG/F-ph, and pPE38muTAAG/R-ph was compared to transcription of the wild-type pe38 (pPE38-CAT82) and polyhedrin (pph-CAT101) promoters in extracts of uninfected cells (u) in the absence of α-amanitin (− α-ama) or in extracts of AcMNPV-infected cells prepared at 20 and 40 h p.i. by the method of Gorski et al. (11), in the presence of α-amanitin (+ α-ama). The extended products are indicated by arrowheads and detailed in Fig. 4.