FIGURE 1.

Identification of SNRPG, OST4, TOMM7, NOP10 and their evaluation versus established cell line and tissue reference genes (RGs) in cell lines of diverse origin. (a) Evaluation of the presence of identified (EV) and established (cell lines and tissue) RGs in five publicly available EV RNA datasets. Heatmap is based on relative abundance within each respective study. (b) Selected panel of 15 cell lines for the evaluation of RGs. (c) Expression profiles obtained by plotting the Cq value deviations relative to the serum condition (ΔCq serum free = avg Cq (RG) in serum – avg Cq (gene) in serum free; ΔCq opti‐MEM = avg Cq (gene) in serum free – avg Cq (gene) in opti‐MEM; data was normalized to average Cq value per RG in serum). Cell lines were cultured for 24 h under three different conditions: 10% serum (circles); serum‐ free (squares) and in Opti‐MEM medium (inverted triangle). Results are represented as expression profiles of tested genes in average delta Cq values after analysis of three technical replicates. CP, chronic pancreatitis; PDAC, pancreatic ductal adenocarcinoma.