Table 2.
Phenolic composition of PE and PE-loaded liposomes by LC-MS/MS method, ionization mode ESI(-)
| Compounds | Molecular formula | RT, min | Precursor ion m/z | Product ion(s) m/z | Cone voltage, V | Collision energy, eV | PE content, mg/g | Content, μg/mL liposome | |
|---|---|---|---|---|---|---|---|---|---|
| L1-PE4 | L2-PE2 | ||||||||
| 3,4 dimethoxy cinnamic acid | C11H12O4 | 19.1 | 206.7 | 102.7 | 25 | 20 | 6.62±0.47 | 440.88±8.64a | 207.72±4.07b |
| Apigenin | C15H10O5 | 25.0 | 269 | 117.3/149/151 | 40 | 30/25/25 | 3.30±0.14 | 187.22±6.52a | 88.21±3.07b |
| Caffeic acid | C9H8O4 | 10.9 | 179 | 135 | 25 | 20 | 4.19±1.15 | 385.78±11.69a | 181.76±5.50b |
| CAPE | C17H16O4 | 32.4 | 283 | 179/161/135 | 25 | 20 | 12.66±0.74 | 649.48±1.41a | 306.01±0.66b |
| Chrysin | C15H10O4 | 30.9 | 253 | 225/209/151 | 25 | 20 | 9.31±1.08 | 450.72±11.54a | 212.36±5.44b |
| Trans-ferulic acid | C10H10O4 | 14.7 | 193 | 178/149/134 | 25 | 20 | 1.60±0.21 | 131.58±8.66a | 61.99±4.08b |
| Galangin | C15H10O5 | 31.9 | 269 | 197/213/227 | 25 | 20 | 14.36±1.48 | 824.20±3.25a | 388.33±1.53b |
| Kaempferol | C15H10O6 | 25.4 | 285 | 93/151/257 | 25 | 20 | 3.68±0.15 | 208.97±24.57a | 98.45±11.57b |
| Luteolin | C15H10O6 | 22.3 | 285 | 133/241/267 | 25 | 20 | 0.94±0.02 | 61.80±10.78a | 29.12±5.08b |
| Naringenin | C15H12O5 | 24.2 | 271 | 145/151 | 25 | 20 | 2.07±0.06 | 106.18±8.01a | 50.03±3.78b |
| p-coumaric acid | C9H8O3 | 13.5 | 163 | 93/119/147 | 25 | 20 | 2.14±0.27 | 191.21±3.92a | 90.09±1.85b |
| Pinobanksin | C15H12O5 | 24.3 | 271.2 | 153/225/253 | 25 | 20 | 14.06±0.98 | 616.90±34.34a | 290.66±16.18b |
| Pinocembrin | C15H12O4 | 31.1 | 255 | 151/171/213 | 25 | 20 | 18.64±0.54 | 1291.69±3.12a | 608.58±1.47b |
| Quercetin | C15H10O7 | 22.4 | 301 | 150.8/178.9 | 35 | 20 | 1.67±0.18 | 151.02±4.29a | 71.15±2.02b |
| Trans-cinnamic acid | C9H8O2 | 21.1 | 147 | 77/102.8 | 25 | 20 | 2.33±0.12 | 183.47±28.99a | 86.44±13.66b |
| Total | 97.56 | 6012.19 | 2782.96 | ||||||
Data are expressed as mean ± S.D. of triplicate measurements. The values with different superscript lowercase letters between L1-PE4 and L2-PE2 samples are significantly different (p<0.05). RT: retention time. PE: propolis extract; L1-PE4: PE (4 %, w/v) loaded liposomes incorporated with T80; L2-PE2 : PE (2 %, w/v) loaded liposomes incorporated with AMP. T80: Tween 80, polyoxyethylene sorbitan monooleate; AMP: ammonium phosphatide 4455.