FIG. 1.
Antibodies (Ab) against the 3′ end of BHV-1 UL10 immunoprecipitate the UL10 in vitro translation product. (A) A 30-kDa protein was synthesized in a reticulocyte lysate in the presence but not the absence of the UL10 RNA transcript. The sample was treated at 56°C for 10 min in the presence of reducing agent, subjected SDS-PAGE in a 12% gel, and autoradiographed. (B) Sera from mice immunized with gMC-63 precipitated the 30-kDa protein synthesized in an in vitro translation reaction programmed with UL10 mRNA. Sera from mice immunized with GST did not precipitate the 30-kDa protein.