FIG. 5.
Pulse-chase and immunoprecipitation to show the maturation of BHV-1 gM. Proteins from 35S-labeled BHV-1-infected cells without (lanes 1 to 4 and 8 to 11) or with (lanes 5 and 12) tunicamycin treatment (Tunic), and similarly labeled uninfected cells (lanes 6 and 13), were chased into mature proteins for 0 to 60 min with unlabeled amino acids. gM was immunoprecipitated with gMC antibody and analyzed by SDS-PAGE on 10 to 20% gradient gels in the presence (lanes 1 to 7) or absence (lanes 8 to 14) of the reducing agent DTT. Unprecipitated UL10 in vitro translation products were analyzed in lanes 7 and 14. The strong signal at 8 to 10 kDa in the UL10 in vitro translation reaction was not present in reactions done in other reticulocyte lysates.