FIG. 7.
Immunoprecipitation of the gM-UL49.5 complex with antibody against either gM or UL49.5. Lysates of radiolabeled uninfected cells (Un), BHV-1-infected cells (Cl), cell membranes (Cm), 40K cell membrane supernatants (Cs), and semipurified virions (V) were precipitated with antibody (Ab) against truncated UL49.5T or gM. Uninfected cells (lanes 1 and 10) were immunoprecipitated with antibodies against both gM and UL49.5. The immunoprecipitates were incubated at 56°C in the presence (lanes 1 to 9) or absence (lanes 10 to 18) of DTT, analyzed by SDS-PAGE in 10 to 20% gradient gels, and autoradiographed. Positions of molecular mass markers (left) and calculated molecular masses of antibody-precipitated bands (right), both in kilodaltons, are indicated. Ag, antigen.