FIG. 3.

Isolated LysRSΔN90, expressed by c2.10, is active in aminoacylation assays. (A) Coomassie blue-stained gel of purified GST-lysRSΔN90. (B) Aminoacylation assay in which the above protein was used to catalyze the reaction between [¹⁴C]lysine and tRNA^Lys. TCA-precipitable radioactivity was measured with a scintillation counter. The background, determined by activity in the absence of tRNA^Lys, was subtracted from the experimental values.