FIG. 2.
Scanned digital images of immunoblots probed with anti-UL15-MBP antibody. Electrophoretically separated proteins were transferred to nitrocellulose and probed with the antiserum directed against the UL15-MBP fusion protein. (A) Type B capsids were purified from cells infected with wild-type HSV-1(F) or with HSV-1(ΔUL15), lacking most of the UL15 second exon. (B) B capsids were purified from cells infected with HSV-1(F) or with HSV-1(R7244), containing a 20-codon epitopic tag at the 3′ end of the UL15 gene. The positions of the bands corresponding to the 83,000- and the 85,000-Mr UL15 proteins (83 and 85, respectively) are indicated.