TABLE 1.
Percentages of capsid proteins remaining after extraction with GuHCla
| Protein | % Protein after extraction with indicated concn (M) of GuHCl
|
||||
|---|---|---|---|---|---|
| 0.05 | 0.1 | 0.5 | 1.0 | 2.0 | |
| VP5 | 100.0 | 100.0 | 93.0 | 79.0 | 40.0 |
| VP19c | 95.0 | 81.0 | 67.0 | 40.0 | 21.0 |
| VP21 | 97.0 | 68.0 | 49.0 | 16.0 | NDb |
| VP22a | 96.0 | 68.0 | 74.0 | 12.0 | ND |
| VP23 | 89.0 | 60.0 | 54.0 | 35.0 | 30.0 |
| VP24 | 92.0 | 58.0 | 37.0 | 3.0 | ND |
| UL15 protein | 81.0 | 83.0 | 75.0 | 16.0 | 15.0 |
a
Percentages were calculated by dividing densitometric values of Coomassie blue-stained proteins in capsids extracted with GuHCl by values of corresponding proteins in untreated capsids. For the UL15 protein, densitometric values of the immunoblot reacted with the UL15-MBP antibody were used.
b
Nondetectable.