TABLE 2.
Detection of positive (+) and negative (−) strands of HGV RNA in serum samples and various tissues from four patients with AIDS
| Patient | HGV RNA titera in:
|
|||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Serum (genomic eq/ml)
|
Tissue (genomic eq of RNA/μg)
|
|||||||||||||||||||||||||
| Liver
|
Spleen
|
Bone marrow
|
Lymph node
|
Pancreas
|
Thyroid
|
Adrenal gland
|
Kidney
|
Lung
|
Muscle
|
Skin
|
Spinal cord
|
|||||||||||||||
| + | − | + | − | + | − | + | − | + | − | + | − | + | − | + | − | + | − | + | − | + | − | + | − | + | − | |
| 1 | 5 × 104 | N | P | N | ND | ND | 103 | 102 | 103 | N | 102 | N | 101 | N | 101 | N | 101 | N | 102 | N | 101 | N | ND | ND | 101 | N |
| 2 | 5 × 104 | N | 102 | N | 103 | 102 | 105 | 103 | 101 | N | 101 | N | P | N | P | N | 101 | N | 101 | N | N | N | 101 | N | N | N |
| 3 | 5 × 105 | N | 103 | 102 | 103 | 102 | 103 | P | 101 | N | P | N | P | N | 101 | N | 101 | N | 101 | N | N | N | 101 | N | N | N |
| 4 | 5 × 104 | N | 102 | N | ND | ND | 102 | N | 103 | N | 102 | N | 101 | N | 102 | N | P | N | 102 | N | 101 | N | ND | ND | N | N |
a
The presence and titers of the positive strand were determined by the MMLV RT-based assay, while the presence and titers of the negative strand were determined by the Tth-based assay. N, negative; P, positive in 6 μg of total RNA; ND, not done.