TABLE 1.
Primer pool | Orientation | Sequence (5′ to 3′)a | Degeneracyb | Length (nt) |
---|---|---|---|---|
PQGWA | Sense | TACCAGTGGAACGTTCTGCCNCARGGNTGG | 32 | 30 |
PQGMA | Sense | TACCAGTGGAACGTTCTGCCNCARGGNATG | 32 | 30 |
PQGFA | Sense | TACCAGTGGAACGTTCTGCCNCARGGNTT | 32 | 29 |
YMDDB | Antisense | GTAGTCTGATCCTACCAACADRTCRTCCATRTA | 24 | 33 |
N = A, T, C, G; R = A, G; D = A, G, T. Residues marked in bold and underlined represent the unique residues which code for either W, M, or F. These residues define the specificity of the oligonucleotide pool.
The number of different primers in the pool representing all codon possibilities at the degenerate positions indicated by N, R, or D.