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. 1998 Apr;72(4):3082–3087. doi: 10.1128/jvi.72.4.3082-3087.1998

TABLE 1.

Primers used to isolate novel retroviral sequences

Primer pool Orientation Sequence (5′ to 3′)a Degeneracyb Length (nt)
PQGWA Sense TACCAGTGGAACGTTCTGCCNCARGGNTGG 32 30
PQGMA Sense TACCAGTGGAACGTTCTGCCNCARGGNATG 32 30
PQGFA Sense TACCAGTGGAACGTTCTGCCNCARGGNTT 32 29
YMDDB Antisense GTAGTCTGATCCTACCAACADRTCRTCCATRTA 24 33
a

N = A, T, C, G; R = A, G; D = A, G, T. Residues marked in bold and underlined represent the unique residues which code for either W, M, or F. These residues define the specificity of the oligonucleotide pool. 

b

The number of different primers in the pool representing all codon possibilities at the degenerate positions indicated by N, R, or D.