TABLE 3.
Reverse transcriptase activity in cells exposed to PoEV isolated from the NIH miniature pig or the Yucatan pig
| Target cell origin | Cpm 3H[TTP] incorporateda
|
|
|---|---|---|
| ST - NIHb | ST - Yc | |
| Mus dunni tail fibroblast | Bkgd | NDe |
| Rat fibroblast (Rat2) | Bkgd | ND |
| Mink lung (CCL-65) | Bkgd | 4,026 |
| Bovine (CCL-22, MDBK) | ND | Bkgdd |
| Hamster lung (E36) | Bkgd | Bkgd |
| Swine testis (ST-IOWA) | 11,515 | 10,919 |
| Human cervical cancer (HeLa) | 5,382 | ND |
| Human embryonic kidney (293) | 18,432 | 27,622 |
Samples of cell culture supernatant were removed 22 days after initiation of coculture and assayed for reverse transcriptase activity, as described in Materials and Methods. Values represent the counts per minute of 3H[TTP] incorporated in coculture sample, with subtraction of background values from matched cultures not exposed to ST-NIH miniature-pig cells.
ST-NIH miniature-pig cells were lethally irradiated and cocultured with target cells as described in Materials and Methods.
Target cells were exposed to culture supernatant from productively infected ST-Y cells.
Bkgd, value measured in coculture sample was less than twofold greater than background value.
ND, not determined.