Figure 1.

(a) Scheme of the recombinant PreS-RBD fusion proteins. RBD domains from hu-1 (green) and from Omicron (orange) were fused to the N- and C-terminus of PreS (blue) as indicated. (b) Coomassie blue-stained SDS-PAGE of HEK cell-expressed and purified PreS-RBD fusion proteins. Molecular weights are indicated in kDa on the left margin. (c) MALDI analyses of fusion proteins. Y-axes: relative intensity as percentage of most abundant signal intensity. X-axes: mass/charge ratio. (d) Melting curve of PreS-RBD fusion proteins. The melting curves are shown forPreS-RBD fusion proteins as percent unfolded protein (y-axes) at increasing temperatures in °C (x-axes).