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. 2024 Feb 23;12(3):229. doi: 10.3390/vaccines12030229

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Secretion of RBD-specific cytokines of supernatants from cultured splenocytes of mice immunized with 20 μg (circles) or 40 μg (triangles) of W-PreS-O, after stimulation with RBD-hu-1, RBD-Omicron or medium. The stimulation indices of cytokines are shown (y-axes; IL-2, IL-4, IL-5, IL-10, IL-12, GM-CSF, IFN-γ, TNF-α) specific for wild-type RBD (RBD-hu-1) or Omicron RBD (RBD-O) (x-axes), calculated as the average measurement of cytokines divided by the average of triplicate of unstimulated cells. Horizontal bars represent median values of groups.