TABLE 2.
Viability and antigen staining of cells in the persistently infected MA104/SA11-B culture
| Day of culture maintenance | Passage no. | Trypan blue exclusiona (%) | Immunocytochemical stainingb (%) |
|---|---|---|---|
| 90 | 6 | 72 | 63 |
| 144 | 10 | 100 | — |
| 153 | 11 | — | 52 |
| 163 | 12 | 95 | — |
| 173 | 13 | 85 | — |
| 194 | 15 | 86 | 42 |
| 210 | 16 | 87 | — |
| 248 | 20 | 100 | — |
| 330 | 28 | 96 | 38 |
a
Cells were mixed 1:4 (vol/vol) with 0.1% trypan blue, incubated at room temperature for 5 to 10 min, and enumerated using a hemacytometer. The percentage of cells not stained with trypan blue was determined by counting 100 cells. —, not done.
b
Viral antigen was detected with an immunoperoxidase assay using a monoclonal antibody directed against VP6. The percentage of cells containing rotavirus antigen was determined by counting approximately 1,000 cells.