FIG. 5.

Sucrose gradient analysis of M-PMV gag and gag-pro programmed in vitro translations. Equal volumes of gradient fractions were separated by SDS-PAGE (10% polyacrylamide gel) and visualized by fluorography. Lane numbers represent the gradient fractions beginning from the top (lane 1). Lane L contained an equivalent aliquot of the translation reaction mixture which was loaded onto each gradient; lane P contained the material which had pelleted in the tube. The electrophoretic positions of the molecular mass protein markers are indicated in kilodaltons on the left, and the positions of Pr78^gag and Pr95^gag-pro are shown on the right. The translation reactions which were treated with ATPγS (top panel) or H₂O (control; bottom panel) are indicated.