. Author manuscript; available in PMC: 2024 Mar 28.

Published in final edited form as: J Chem Inf Model. 2023 Nov 10;63(22):7180–7188. doi: 10.1021/acs.jcim.3c01269

Table 2. Omicron BA.1 SARS-CoV-2 replicon with the S144A/E166A 3CLpro mutation has decreased susceptibility to nirmatrelvir.

Susceptibility of SARS-CoV-2 replicons to nirmatrelvir was assessed by Nanoluc activity. EC₅₀ mean and standard deviations (SD) are determined from using both technical (n = 2) and biological (n = 2) replicates.

Omicron BA.1 strain (3CLpro variant)	EC₅₀ ± SD (μM) (Fold change from WT BA.1)
WT_{Omicron BA.1}	0.11 ± 0.02 (1)
(S144A/E166A)_{Omicron BA.1}	2.22 ± 0.41 (20)