FIG. 5.

Mutations in the HCF-binding motif do not affect the activation domain of Luman. Luman and its mutants were fused to the GAL4 DBD to study the effects of the mutations on their ability to activate transcription when tethered to the GAL4 UAS. Equivalent amounts (0.5 μg) of each GAL-Luman fusion protein-expressing plasmid were cotransfected in COS7 cells with plasmid pG5EC (0.5 μg) as the reporter. The control represents the expression vector without an insert.