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A mutation in the VP16-binding site of HCF(P134S) prevents its association with VP16 as well as with Luman and dCREB-A in vitro. GST and GST fusion proteins of dCREB-A, Luman, and VP16 were incubated with an equivalent amount of 35S-labeled HCF (A) or HCF(P134S) (B) and analyzed on an SDS-polyacrylamide gel. Input (1/10) was run on the last lane of each gel.
