Skip to main content
. 2024 Mar 5;4(3):1194–1206. doi: 10.1021/jacsau.4c00009

Figure 1.

Figure 1

Characterization of PSFG properties and bimodal signal changes after the reaction with GSH. (A) Particle sizes of different polymeric micelles. (B) TEM images of (a) PSF, (b) PSG, (c) PSFG micelles in deionized water, and (d) PSFG micelles disassembled with GSH. Scale bars in (a)–(d) = 200 nm. Inset in (c): HR-TEM image of PSFG, scale bar = 50 nm. GSH concentration-dependent (C) absorption spectra and (D) fluorescence spectra of PSFG in PBS (10 mM, pH 7.4) after 10 h at 37 °C with λex = 776 nm. (E) Concentration-dependent 19F NMR spectra of PSFG with the mass fractions of PSG components in the entire PSFG micelle (0, 20, 40, 60, 80, and 100%). 50% DMSO + 40% H2O+ 10% D2O as solvent. CF3COONa, with a peak of 19F at −73.6 ppm, was used as an internal reference. (F) Time-dependent 19F NMR spectra of PSFG (5 mg mL–1) after the addition of GSH (10 mM) at 37 °C in PBS. (G) In vitro drug release of PSFG micelles in 10 mM GSH. The red and blue curves represent the GEM release results fitted by HPLC and 19F NMR, respectively. (H) GSH concentration-dependent fluorescence intensity (black) at low GSH concentrations (≤0.25 mM) and GSH concentration-dependent 19F NMR (red) at high GSH concentrations (1–10 mM). The black and red lines represent the linear fitting of the data.