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. 2024 Mar 5;4(3):1194–1206. doi: 10.1021/jacsau.4c00009

Figure 2.

Figure 2

In vitro imaging of the PSFG micelle activity in tumor cells. Confocal laser scanning microscopy (CLSM) imaging of A549 cells incubated with PSFG micelles (100 μg mL–1) (A) at different time points (1, 2, 4, and 8 h) and (B) corresponding fluorescence intensity; scale bar = 100 μm. (C) GSH-insensitive PF micelles (100 μg mL–1) served as a control. The fluorescence images were collected in the NIR channel (λem = 808 ± 30 nm, λex = 776 nm, CW laser). Scale bar = 100 μm. (D) CLSM image of A549 cells cocultured with PSFG micelles; scale bar = 50 μm. The scale bars for local magnification are 10 μm. (E) The signal intensity distribution of PSFG (red), Lyso-Tracker (green), and DAPI (blue) channels after treatment with PSFG micelles for 8 h. (F) Fluorescence intensity of intracellular PSFG at 1 h (green), 2 h (orange), 4 h (blue), and 8 h (red) after incubation by flow cytometry analysis. (G) 19F MR phantom images of A549 cells incubated with PSFG micelles (1 mg mL–1) with four cell lines (WI-38, MCF-7, A549, and HeLa) and (H) corresponding 19F MRI signal intensities. (I) PEITC is involved in GSH consumption as a competitive inhibitor.