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. 2024 Mar 5;4(3):1194–1206. doi: 10.1021/jacsau.4c00009

Figure 4.

Figure 4

In vitro and in vivo antitumor effects of PSFG micelles after incubation with A549 cells or subcutaneous injection in male Balb/c nude mice bearing A549 xenografts. (A) Representative images of live (green) and dead (red) assays of A549 cells treated with PSF, GEM, PSG, and PSFG with the same GEM content. Scale bar = 200 μm. (B) Deoxyribonucleic acid (DNA) damage in A549 cells was detected by comet electrophoresis (single-cell gel electrophoresis experiment). Scale bar = 20 μm. (C) Representative images of live (green) and dead (red) assay of A549 cells treated with different PSFG concentrations. Scale bar = 200 μm. (D) Assessment of Cell death mechanism with annexin-V-FITC/PI by flow cytometry. (E) Timeline of the administration of PSFG micelles and control groups to A549 tumors. (F) In vivo antitumor study of PBS, PSF, free GEM, PSG, and PSFG. The variation of tumor volume changes over 21 days after injection. The drugs injected in the three experimental groups in GEM, PSG, and PSFG were measured at the same GEM equivalence (mean ± SD, n = 6, ***p < 0.001). (G) Photographs and (H) weights of tumors after treatment in different groups for 21 days (mean ± SD, n = 6, **p < 0.01).