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. 2024 Mar 5;4(3):1194–1206. doi: 10.1021/jacsau.4c00009

Figure 5.

Figure 5

Safety evaluation of different groups of male Balb/c nude mice bearing the A549 xenograft model. (A) In the hemolysis analysis of micelles at different concentrations, the percent of hemolysis was less than 5%, even at micellar concentrations up to 500 μg mL–1. (B) Damage to normal cellular DNA by PSFG was detected by comet electrophoresis. Scale bar = 20 μm. (C) Body weight changes over 21 days after the injection. (D) Images of H&E assays for the heart, liver, spleen, lungs, kidneys, and tumors after treatment with PBS, PSF, free GEM, PSG, and PSFG micelles over 21 days. Scale bar = 100 μm. (E) Immunohistochemical (IHC) analysis of tumor sections (CD31, Ki67, and TUNEL). Scale bar = 100 μm. (F) Immunofluorescence images of tumor tissues stained for CD4+, CD8+, and PD-L1 on day 21 (n = 7, all tissues: 100×). Scale bar = 100 μm.