FIG. 5.

Induction of apoptosis in HVS-transformed T cells by CD95 ligation. The HVS-transformed T-cell clone SS-BP8T was treated with 2 μg of MAb CH-11 per ml, and 10⁷ T cells were collected at each indicated time after the addition of CH-11. The method used to obtain fragmented DNA includes a mild detergent lysis that leads to an enrichment of apoptotic DNA. The DNA in the right lane was obtained from untreated SS-BP8T (neg). The DNA fragmentation after treatment with MAbs to CD95 was visualized on an agarose gel. The DNA size marker is on the left; sizes are given in kilobases.