TABLE 1.
Effects of CsA, PGE2 dexamethasone, irradiation, and CD95 ligation on HVS-transformed T cell clones
| Treatment | Inhibition of proliferation (%)a | Specific cell death (%)b | Cell cycle distribution (%)c
|
||
|---|---|---|---|---|---|
| G1 | S | G2 | |||
| None (Control) | 0 | 76 ± 2 | 16 ± 2 | 8 ± 1 | |
| Irradiation (3,000 rads) | 99 ± 0 | 34 ± 4 | 91 ± 2 | 6 ± 2 | 3 ± 1 |
| CsA (1 μg/ml) | 73 ± 7 | 11 ± 3 | 85 ± 1 | 10 ± 1 | 5 ± 1 |
| Dexamethasone (10−3 M) | 84 ± 4 | 33 ± 7 | 84 ± 0 | 12 ± 0 | 4 ± 0 |
| PGE2 (10−4 M) | 100 ± 0 | 4 ± 3 | 87 ± 2 | 8 ± 3 | 5 ± 1 |
| Anti-CD95 (2 μg/ml) | 70 ± 5 | 74 ± 5 | NDd | ND | ND |
To measure proliferation, [3H]thymidine was added 24 h (anti-CD95) or 48 h (all other treatments) after initiation of the treatments for another 16 h. Values are means and standard errors of the mean (SEM) for 6 to 12 experiments.
The specific cell death was determined as described in Materials and Methods 24 h (anti-CD95) or 48 h (all other treatments) after initiation of the treatments. Values are means and SEM for 6 to 12 experiments.
The cell cycle distribution of the viable cells was quantified 48 h after treatment by measuring the PI uptake by the fixed and permeabilized cells as described in Materials and Methods. Values are means and SEM for three experiments.
ND, not determined.