FIG. 2.

Intracellular stability (A) and transport (B) of GP₃ protein expressed during IAF-Klop infection of MARC-145 cells. Infected cells were pulse-labelled with [³⁵S]methionine for 15 min and chased in the presence of 5 mM nonradioactive methionine for the indicated periods. Cell lysates were immunoprecipitated with α3 antiserum and were then subjected to treatment with Glyco F or Endo H or were left untreated. Numbers on the right indicate the positions and sizes of marker proteins. The arrows show the two protein bands resulting from incomplete deglycosylation. Since they were also generated by Glyco F digestion, they must not be considered partially resistant to Endo H.