Table 1. Substitutions of Amino Acid Residues Between Genogroups A and B of CVB5, and Description of Mutants Produced in this study.

			genogroup A		genogroup B
protein	residuea	locationb	(n = 7)	Faulkner	(n = 6)	at the capsid surface	Met invloved	mutants generated in this study	name of mutantc
VP1	19	interior	G	G	S
VP1	95	interior	S	N	N
VP1	156	EF loop	V	V	I	x		x	CVB5F.cas.VP1.V156I
VP1	180	buried, hydrophobic pocket	M	M	I		x	x	CVB5F.cas.VP1.M180I
VP1	276	C-terminal	D	D	E	x		x	CVB5F.cas.VP1.D276E
VP1	279	C-terminal	T	T	A	x		x	CVB5F.cas.VP1.T279A
VP2	37	interior	V	V	T
VP2	45	interior	D	D	E
VP2	137	EF loop (puff)	L	L	I	x		x	CVB5F.cas.VP2.L137I
VP2	156	EF loop (puff)	E	E	D	x		x	CVB5F.cas.VP2.E156D
VP2	160	EF loop (puff)	S	S	T	x		x	CVB5F.cas.VP2.S160T
VP2	260	C-terminal	K	K	R	x		x	CVB5F.cas.VP2.K260R
VP3	35	interior	E/N/Dd	E	A
VP3	63e	N-terminal helix close to β-strand B (knob)	M/Ld	M	S/Td	x	x	x	CVB5F.cas.VP3.M63L
VP3	67f	N-terminal helix close to β-strand B (knob)	S/A	A	A
VP3	88	CD loop	T/M/Id	T	I	x		x	CVB5F.cas.VP3.T88I
VP4	17	disordered	L	L	V
VP4	45	interior	D	D	E
VP4	47	interior	T	T	A
VP1–4	all of the above excepte,f								CVB5F.cas.genogroupB

^aThe numbering of amino acids is according to the residue positions in the CVB5 Faulkner.⁶³

^bThe location was assigned according to previous studies.^40,64

^cCVB5F.cas corresponds to the Faulkner strain with a ClaI restriction site introduced at a non-structural protein region (nucleotide position 3340).

^dMultiple amino acid residues were observed within the genogroup.

^eStrains belonging to genogroup B do not possess single specific amino acids.

^fThe same amino acid residue is observed for CVB5 Faulkner and strains belonging to genogroup B.