FIG. 3.

Gel filtration analysis of e55K through Superose 6 (A) and Superose 12 (B) columns. After chromatography, fractions were trichloroacetic acid precipitated and analyzed by SDS-PAGE and Western blotting with the anti-55K antibody 2A6. e55K was visualized by ECL. Fraction numbers are indicated at the bottom of each panel. The fractions in which the protein standards eluted are indicated at the top of each panel. Protein standards were thyroglobulin (a tetramer of 670 kDa [85Å]), apoferritin (a multimer of 440 kDa [61Å]), β-amylase (200 kDa), ADH (a tetramer of 150 kDa [46Å]), BSA (a monomer of 68 kDa [35Å]), and carbonic anhydrase (29 kDa).