Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Nov 22;44(13):e0374232023. doi: 10.1523/JNEUROSCI.0374-23.2023

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2024 Lombino et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

PMC Copyright notice

Figure 3. — p60 and p80 katanin are located at excitatory spine synapses. A, Immunostaining of endogenous katanin p80 (green) and Rhodamine phalloidin labeling of F-actin (red), DIV13 neurons, three independent experiments. B, Pearson's correlation coefficient indicating colocalization of p80 katanin and F-actin. Mean ± S.E.M = 0.5149 ± 0.01379, n = 25 ROIs. C, Magnifications of spines from boxed regions in A. D, Fluorescence intensity profiles of line scans in C. E, Co-immunostaining of endogenous p80 katanin (green) and AMPAR subunit GluA2 (red), DIV13–16 neurons, three independent experiments. F, Magnification of boxed regions in E. G, Fluorescence intensity profiles of line scans in F. H, Western blot analysis of p60 katanin in Triton-X-100-soluble (TS) and Triton-X-100-insoluble (TI) synaptosomal fraction. The TI fraction is also known as PSD fraction, which is enriched for postsynaptic markers (PSD-95, GluA1), but contains very little presynaptic marker (SNAP-25). n = 3 experiments. I, Anti-p60 katanin immunoelectron microscopy with diaminobenzidine (DAB) showing a katanin-negative (left) next to a katanin-positive (right, red arrow) spine synapse from hippocampal neurons.