Figure 6.

Inhibition of katanin function interferes with glutamate-induced structural spine remodeling. A, Glutamate uncaging at single synapses using cultured hippocampal neurons that express either GFP or GFP-p60DEID. Left: before stimulation (black), right: after stimulation (green). Red dot: time point of uncaging stimulus. B, Quantification shows a significant difference in spine size (Δ spine size) between GFP and GFP-p60DEID after stimulation. two-way ANOVA test, significant interaction between time and condition, p < 0.0001, multiple comparison with Fisher's LSD test shows significant effects “after 8 min” p = 0.0401; “after 17 min” p = 0.0132; “after 18 min” p = 0.0393 (GFP, n = 39 spines from 14 cells; GFP-p60DEID n = 45 spines from 16 cells), 2 min bins. Before uncaging 10–0 min (black axis labels), after uncaging 0–20 min (green axis labels), five independent experiments. Individual p-values of B: before 10: p = 0.2037, before 9: p = 0.3285, before 8: p = 0.643, before 7: p = 0.4352, before 6: p = 0.4912, before 5: p = 0.8337, before 4: p = 0.4603, before 3: p = 0.9628, before 2: p = 0.1799, before 1: p = 0.2105, after 1: p = 0.8589, after 2: p = 0.1784, after 3: p = 0.0958, after 4: p = 0.1491, after 5: p = 0.1095, after 6: p = 0.0886, after 7: p = 0.1137, after 8: p = 0.0401, after 9: p = 0.0694, after 10: p = 0.0904, after 11: p = 0.1002, after 12: p = 0.4043, after 13: p = 0.3584, after 14: p = 0.0993, after 15: p = 0.0769, after 16: p = 0.1022, after 17: p = 0.0132, after 18: p = 0.0393, after 19: p = 0.0591, after 20: p = 0.3665.