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. 2023 Nov 22;44(13):e0374232023. doi: 10.1523/JNEUROSCI.0374-23.2023

Figure 8.

Figure 8.

Functional inhibition of katanin alters synaptic transmission. A, Katanin GFP-p60DEID overexpression in primary hippocampal neurons does not alter mEPSCs under basal conditions, GFP: n = 21 neurons, GFP-p60DEID: n = 12 neurons, three independent experiments. B, Mean amplitudes (GFP: 15.03 ± 1.26 pA; GFP-p60DEID: 14.57 ± 1.21 pA, Mann–Whitney U test p = 0.9853. C, Medians of inter-event intervals (IEI). GFP: 1,952 ± 565.2 ms; GFP-p60DEID: 1,780 ± 736.3 ms, Mann–Whitney U test p = 0.5181. D, Katanin p60DEID overexpression in primary hippocampal neurons suppresses synaptic potentiation (cLTP). three independent experiments. E, EPSC amplitudes (pA). Stimulation increases the amplitudes of control transfected cells. GFP control, directly after cLTP induction: 128%; GFP control, 30 min after cLTP induction: 139%; GFP-p60DEID, directly after cLTP induction: 108%; GFP-p60DEID, 30 min after cLTP induction: 99%. 2-way ANOVA: time × condition interaction p = 0.0088. Individual data points t tests: 10 min: p = 0.0478; 30 min: p = 0.0464. 5 min bins. F, Inter-event intervals (IEIs). two-way ANOVA: time × condition no interaction p = 0.1925. Bins: 5 min. Red bar: time point of cLTP stimulus.