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. 2024 Feb 28;134(7):913–930. doi: 10.1161/CIRCRESAHA.122.321292

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© 2024 The Authors.

Circulation Research is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial-NoDerivs License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited, the use is noncommercial, and no modifications or adaptations are made.

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Figure 8. — Cardiomyocyte-specific deletion of ROBO1 reduces cardiac hypertrophy and dysfunction after transverse aortic constriction (TAC). A, Schematic of Robo1^fl/fl mouse model and PCR analysis for quantitation of loxp-flanked Robo1 gene region in wild-type (WT), heterozygous (loxp/-), or homozygous (loxp/loxp) mice. B, Immunofluorescence staining using anti-cardiac troponin (c-TNT) antibody (red), anti-ROBO1 antibody (green), and 4’,6-diamidino-2-phenylindole (DAPI; blue) of heart sections from Robo1^fl/fl control or Myh6-CreERT2;Robo1^fl/fl mice. C, B-mode and M-mode echocardiographies of Robo1^fl/fl or Myh6-CreERT2;Robo1^fl/fl mice after sham or TAC surgery with analysis of ejection fraction (EF), fractional shortening (FS), relative end-diastolic left ventricle posterior wall thickness (LVPWd), and relative left ventricle mass. N=8 mice/group. D, Explanted hearts from Robo1^fl/fl and Myh6-MerCreMer;Robo1^fl/fl mice after sham or TAC surgery (top); representative images of heart sections stained with hematoxylin & eosin (H&E; scale bar, 500 µm; middle); and representative images of wheat germ agglutinin (WGA)–stained heart sections (scale bar, 20 µm; bottom). E, Heart weight to body weight (HW/BW) ratio in Robo1^fl/fl and Myh6-MerCreMer mice after sham or TAC surgery. N=8 mice/group. F, Quantification of myocyte cross-sectional area on WGA staining. G, Quantitative PCR (qPCR) analysis of transcription of hypertrophy-related genes (Nppa, Nppb, Myh7, and Acta1, normalized to Gapdh mRNA levels) in hearts from Robo1^fl/fl or Myh6-MerCreMer;Robo1^fl/fl mice after sham or TAC surgery. Two-way ANOVA and the Tukey multiple comparisons test were used for all comparisons.