FIG. 1.

Protein expression in virion and cell lysates. 293T cells were transiently transfected with MLV Gag-Pol and genome constructs with (+ EBO) or without (− EBO) pCB6-Ebo-GP, a Zaire subtype Ebo-GP expression construct. Viral supernatants were harvested from transfected cells and partially purified by pelleting through 20% sucrose. Cell lysates and lysed pellets were analyzed by Western blotting with anti-Ebo-GP serum and anti-MLV capsid serum. (A) Expression of Ebo-GP in cell lysates. Arrows indicate the two forms of Ebo-GP. (B) Incorporation of Ebo-GP into MLV particles. (C) Analysis of MLV capsid in viral pellets. Arrows indicate MLV Gag precursor (Pr65) and capsid (p30) proteins. The positions of molecular mass markers (in kilodaltons) are shown on the left of each gel.