TABLE 2.
Concentration of MLV(Ebola) by centrifugation
| Expt | Virus titer (IU/ml)a
|
Fold concn | Total virus (IU)
|
% Recovery | ||
|---|---|---|---|---|---|---|
| Preconcn | Postconcn | Preconcn | Postconcn | |||
| 1 | 5.9 × 104 | 5.4 × 106 | 78 | 4.6 × 106 | 3.8 × 106 | 82.6 |
| 2 | 1.1 × 105 | 6.2 × 106 | 70 | 7.7 × 106 | 6.2 × 106 | 80.5 |
a
Titers of the concentrated MLV(Ebola) were determined on 293T cells. Preconcentration titer refers to viral supernatant left overnight at 4°C before being used for infection. Postconcentration titer refers to viral supernatant centrifuged at 25,000 rpm in an SW28 rotor for 90 min and resuspended in TNE overnight at 4°C before being used for infection.