Fig. 6. TPA particle-mediated targeted gene delivery in Huh-7 spheroid culture. (A) Secreted luciferase (lucia) activity from cultured media of transduced Huh-7 spheroid culture. TPA-lucia at 1 × 106 TU per cell was used for transduction. (B) Cell viability of transduced Huh-7 spheroid culture. TPA-tmTRAIL at 1 × 106 TU per cell was used for transduction. Cell viability was determined on day 7 post transduction using the MTT assay. All results are shown as mean ± SD. All experiments were conducted in triplicate. *P < 0.05, **P < 0.01 and ***P < 0.001.
