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Effect of EDTA concentration on CNA1 mRNA amplification by RT-PCR. RNA samples extracted from cells harvested at 1.2 mg (dry weight/mL) were treated with DNase I according to protocol II. The reactions were stopped by the addition of 1.25 mM (lane 1), 1.5 mM (lane 2), 1.75 mM (lane 3); 2.0 mM (lane 4) and 2.25 mM (lane 5) of EDTA and treated samples were amplified by RT-PCR using CNA1 primers. P, 100 bp DNA Ladder (BioLabs-Inc).
