Table 1.
Target mRNA | Primer Sequence | Amplicon Size (bp) |
TPS1 |
Forward CGCTAAGGCGCAACTGACCTCGTCT Reverse CGACGAGAATGCGTGGTTGGCATAC |
379 |
TPS2 |
Forward CCCCCCAAACTATCAGATTGGAACAAC Reverse ACCCAGCTGCAGCTATTCCATCGGC |
612 |
PDA1 |
Forward GGTCAGGAGGCCATTGCTGT Reverse GACCAGCAATTGGATCGTTCTTGG |
673 |
CNA1 |
Forward CGAAAGACTTGAATTCTTCACGCATC Reverse GAATGATCTGCAGCAAGCATCG |
629 |
CNA2 |
Forward CCTTATATCTGTTCCCGCCC Reverse GAGGAACCATGGTTTTGGAG |
551 |
ACT1 |
Forward CCTACGTTGGTGATGAAGCT Reverse GTCAGTCAAATCTCTACCGG |
407 |
CNA1, CNA2, PDA1 and ACT1 primer sequences were obtained from Del Aguila et al, 2003 and TPS1 and TPS2, from Souza, 2001.