FIG. 1.

Expression of E1A protein in wt- and mutant-infected mouse 3T6 cells. Mouse 3T6 cells were infected with either wt virus (pmE301) or the indicated mutant virus at an MOI of 5, or they were mock infected. [³⁵S]methionine- and [³⁵S]cysteine-labeled infected cell lysates were harvested at 22 h (early [E]) and 40 h (late [L]) p.i. and immunoprecipitated with an anti-MAV-1 E1A antibody (E1A) or preimmune serum (pre). Immunoprecipitated products were electrophoresed on an SDS–12% polyacrylamide gel followed by phosphorimager analysis. Marker designates ¹⁴C-labeled protein standards, whose sizes are indicated on the left.