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. 2023 Nov 20;38(3):287–300. doi: 10.3233/CBM-220044

Figure 2.

Figure 2.

Inhibition of KRAS reduces SFRP2 expression in PDAC. A, B, C, D) Band densities (average intensity x band surface) were normalized to the loading control, Vinculin, to eliminate inter-sample variability, to quantitatively obtain the Intensity Ratio. For each cell type, results were normalized to the untreated control: -Dox (A), and 0 h (B). (A) Western blot analysis of PDAC expressing a doxycycline (DOX)-inducible CFP-R15 monobody. DOX-induced R15 (+) reduced SRFP2 levels in both PDAC lines and a PANC-1 tumor sample. DOX: doxycycline. (B) Western blot analysis of PANC-1 cells treated with DMSO or MRTX1133 (10 or 20 nM), a noncovalent selective KRASG12D inhibitor, for various periods of time shows a reduction of p-ERK and SFRP2 at 4 hours compared to control. (C) Treatment of MIA PaCa-2 cells with trametinib (MEK inhibitor) at 100 nm for 4 hours shows a reduction in p-ERK and SFRP2. (D) Treatment of MIA PaCa-2 cells with buparlisib (PI3Kinase inhibitor) at 1 uM for 4 hours shows a reduction in p-AKT and SFRP2 compared to control.