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. Author manuscript; available in PMC: 2024 Mar 28.
Published in final edited form as: Benef Microbes. 2021 Sep 22;12(6):583–599. doi: 10.3920/BM2020.0216

Figure 3.

Figure 3.

Limosilactobacillus reuteri promotes serotonin transporter (SERT) at the apical membrane in the setting of TNBS colitis. (A) Representative immunostaining of mouse colon 3 days following TNBS administration in mice treated with either phosphate buffered saline (PBS) (control) or 109 live L. reuteri. SERT is identified in pink, nuclei in blue, and SERT apical localisation is outlined in white. Scale bar = 50 μm. (B) Quantification of mouse colon immunostaining for SERT in TNBS + PBS or TNBS + L. reuteri treated mice using FIJI software. (C) Histological scoring performed by a blinded board-certified pathologist. (D) qPCR analysis of pro-inflammatory cytokines tumour necrosis factor (TNF), chemokine (C-X-C motif) ligand 1 (KC; interleukin-8 homologue), and interferon (IFN)-γ in TNBS + PBS or TNBS + L. reuteri treated mice. * P<0.05, ANOVA. n=6 mice/group.