Figure 2.

Translation of RNA transcripts with two different 5′-UTRs of M2 mRNA in HeLa cells. (A) Schematic representation of the reporter constructs containing the M2 5′-UTR. T7 promoter and the luciferase reporter gene are boxed. The poly(A) tail is shown as A30. Arrows indicate the transcription start site. (B) RNA transcripts of luciferase with different M2 5′-UTRs. The RNAs were generated from linearized reporter expression constructs by in vitro transcription using T7 RNA polymerase. (C) Translation of luciferase reporter with different M2 5′-UTRs. The translation efficiency of luciferase reporter was measured by the determination of the relative luciferase activity in HeLa cells following transfection of the RNA transcripts shown in (B) and normalization to the activity of the co-transfected β-galactosidase.