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. 2005 May 11;33(8):2715–2725. doi: 10.1093/nar/gki569

Figure 6.

Figure 6

Effect of poliovirus protease 2A on stability of luciferase reporter transcripts with different M2 5′-UTRs. In vitro transcripts of luciferase reporters with different M2 5′-UTRs carrying deletions (A and B) or point mutations (C and D) were co-transfected into HeLa cells with transcripts of β-galactosidase 24 h following transfection with pcDNA3 (A and C) or 2Apro expression plasmid (B and C). Total RNAs were then isolated 8 h following RNA transfection for real-time PCR analysis and the relative levels of luciferase RNAs were determined as described in Materials and Methods.