Fig 1. The effect of p53 dosage on Kras-driven tumorigenesis.

(A) Experimental design to study the effect of the Super p53 allele on tumorigenesis upon activating Kras^LSL-comG12D versus Kras^LSL-comQ61R alleles by tamoxifen in a Rosa26^CreERT2/+ background. Mouse and organ images are reprinted from Biorender.com under a CC BY license, with permission from Biorender, original copyright 2023. (B) Kaplan-Meier survival curve of Rosa26^CreERT2/+ mice with the indicated genotypes (day 0 refers to the last tamoxifen injection). Dotted lines: 50% survival. Pairwise comparison of survival curves of each genotype with versus without the Super p53 allele were performed with Gehan-Breslow-Wilcoxon test. *: p = 0.047. Individual survival data values are provided in S1 Appendix. (C) Percent of Rosa26^CreERT2/+ mice of the indicated genotypes (n = 5–8) with the described tumor types. Statistical comparisons of tumorigenesis in each genotype with and without the Super p53 allele were done using One-way ANOVA Bonferroni’s multiple comparison test. *: p = 0.041. Examples of H&E-stained slides of the indicated tissues are provided in S2 Fig. Tumor incidence data values are provided in S2 Appendix.