Figure 4.

Western blot analysis of STAT3 phosphorylation. Western blot analysis of the Epstein-Barr virus transformed lymphoid cell lines (EBV-LCLs) derived from the patients after stimulation with fusion protein of IL-6R and IL-6 (FP6). In addition, the cells were treated with and without either tofacitinib (A) or ruxolitnib (F). (B–E and G–J) Quantification of the protein band intensity (pSTAT3/STAT3 corrected to the GAPDH internal control, and pSTAT3/STAT3 ratio in each sample) was measured using ImageJ software (National Institutes of Health, Bethesda, MD). Asterisks indicate p < 0.05.