Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo sapiens) | A549 | ATCC | CVCL_0023 | |
| Cell line (H. sapiens) | NCI-H23 | ATCC | CVCL_1547 | |
| Cell line (Mus musculus) | MLO-Y4 | ATCC | CVCL_M098 | |
| Cell line (M. musculus) | MLO-A5 | ATCC | CVCL_0P24 | |
| Cell line (M. musculus) | MC3T3-E1 | ATCC | CVCL_0409 | |
| Cell line (M. musculus) | LLC | ATCC | CVCL_4358 | |
| Cell line (H. sapiens) | NCM460 | ATCC | CVCL_0460 | |
| Antibody | Anti-CD63 (Mouse monoclonal) | Abcam | ab193349 | 1:1000 |
| Antibody | Anti-TSG101 (Mouse monoclonal) | Abcam | ab83 | 1:1000 |
| Antibody | Anti-ALIX (Rabbit polyclonal) | Abcam | ab76608 | 1:1000 |
| Antibody | β-Actin (Rabbit monoclonal) |
Cell Signaling Technology | 4970S | 1:4000 |
| Antibody | GAPDH (Rabbit monoclonal) |
Cell Signaling Technology | 2118 | 1:5000 |
| Antibody | MDM2 (Rabbit polyclonal) |
Proteintech | 66511-1-IG | 1:1000 |
| Antibody | Ki-67 (Rabbit monoclonal) |
Cell Signaling Technology | 9129 | 1:200 |
| Antibody | DMP1 (Sheep polyclonal) |
R&D Systems | AF4386 | 1:100 |
| Antibody | GFP (Mouse monoclonal) |
ABclonal | AE012 | 1:100 |
| Antibody | CCND3 (Rabbit polyclonal) |
Bioss | bs-0660R | 1:100 |
| Sequence-based reagent | Cel-miR-39-3p_F | Vazyme | qRT-PCR Forward primer | 5'-GCGTCACCGGGTGTAAATC-3' |
| Sequence-based reagent | mmu-miR-365-1-5p_F | Vazyme | qRT-PCR Forward primer | 5'-AGGGACTTTTGGGGGCA-3' |
| Sequence-based reagent | mmu-miR-99b-3p_F | Vazyme | qRT-PCR Forward primer | 5'-GCGCAAGCTCGTGTCTGTG-3' |
| Sequence-based reagent | mmu-miR-193a-5p_F | Vazyme | qRT-PCR Forward primer | 5'-TGGGTCTTTGCGGGCA-3' |
| Sequence-based reagent | mmu-miR-125a-3p_F | Vazyme | qRT-PCR Forward primer | 5'-CGCGACAGGTGAGGTTCTTG-3' |
| Sequence-based reagent | mmu-miR-690_F | Vazyme | qRT-PCR Forward primer | 5'-GCGAAAGGCTAGGCTCACA-3' |
| Sequence-based reagent | mmu-miR-1195_F | Vazyme | qRT-PCR Forward primer | 5'-GTGAGTTCGAGGCCAGCC-3' |
| Sequence-based reagent | mmu-miR-122-5p_F | Vazyme | qRT-PCR Forward primer | 5'-CGCGTGGAGTGTGACAATGG-3' |
| Sequence-based reagent | mmu-miR-140-3p_F | Vazyme | qRT-PCR Forward primer | 5'-GCGCGTACCACAGGGTAGAA-3' |
| Sequence-based reagent | Reverse primer of all miRNAs | Vazyme | qRT-PCR Reverse primer | 5'-AGTGCAGGGTCCGAGGTATT-3' |
| Commercial assay or kit | PKH67 Green Fluorescent Cell Linker Kit | Sigma-Aldrich | PKH67GL | |
| Commercial assay or kit | Cell-Light EdU Apollo488 In Vitro Kit | Ribobio | C10310-3 | |
| Commercial assay or kit | miRCURY LNA miRNA Detection Kit | Exiqon | 339115 | |
| Chemical compound, drug | GW4869 | Sigma-Aldrich | D1692 | |
| Software, algorithm | GraphPad Prism | GraphPad Software | Version 6.0 RRID:SCR_002798 |