Table 1.

Major species detected by SEC-UV-native MS in mAb1 stability samples at T0 and after 3-month incubation at 25°C (“3M25C”).

Sample name	Retention time (min) and peak naming	Detected mass (Da)	Assigned identity	Theoretical mass# (Da)	% UV peak area
T0	6.3 (HMW)	290.5 k	mAb1 dimer	290,194	0.6
	7.1 (main peak)	145,094	mAb1	145,097	98.0
	7.7 (LMW1)	98,950	Fab/c*	98,958	1.4
3M25C	6.3 (HMW 1)	290.5 k	mAb1 dimer	290,194	1.8 (total HMWs)
	6.5 (HMW 2)	244.5 k	mAb1-Fab/c	244,054
	6.6 (HMW 3–1)	198.5 k	Fab/c dimer	197,915
	6.7 (HMW 3–2)	191.0 k	mAb1-Fab	191,254
	6.9 (main peak)	145,092	mAb1	145,097	95.6
	7.7 (LMW1)	98,960	Fab/c*	98,958	2.1
	8.6 (LMW2)	46,159	Fab*	46,157	0.4

*Masses of Fab/c and Fab fragments were calculated based on cleavage site KSCDKTH/TCPPC.

^#All the theoretical masses of mAb1 and Fab/c were calculated based on two copies of FA2 glycan were included.