Table 2.
Major species detected by SEC-UV-native MS in mAb2 stability samples at T0 and after 3-month incubation at 25°C (“3M25C”).
| Sample Name | Retention time (min) and Peak Naming | Detected mass (Da) | Assigned identity | Theoretical mass (Da) | %UV peak area |
|---|---|---|---|---|---|
| T0 | 6.6 (HMW) | 289.4 k | mAb2 dimer | 288,882 | 0.8 |
| 7.5 (main peak) | 144,441 | mAb2 | 144,441 | 97.4 | |
| 8.0 (LMW1) | 97,118 | Fab/c* | 97,123 | 1.8 | |
| 3M25C | 6.5 (HMW) | 289.4 k | mAb2 dimer | 288,882 | 2.8 |
| 7.4 (main peak) | 144,442 | mAb2 | 144,441 | 94.5 | |
| 8.0 (LMW1) | 97,124 | Fab/c* | 97,123 | 2.1 | |
| 9.3 (LMW2) | 47,335 | Fab* | 47,336 | 0.6 |
*Fab/c and Fab fragments were identified with cleavage site KSCDKTH/TCPPC.